Characterization of a receptor for insect tachykinin-like peptide agonists by functional expression in a stable Drosophila Schneider 2 cell line

J Neurochem. 2000 May;74(5):2182-9. doi: 10.1046/j.1471-4159.2000.0742182.x.


STKR is an insect G protein-coupled receptor, cloned from the stable fly Stomoxys calcitrans. It displays sequence similarity to vertebrate tachykinin [or neurokinin (NK)] receptors. Functional expression of the cloned STKR cDNA was obtained in cultured Drosophila melanogaster Schneider 2 (S2) cells. Insect tachykinin-like peptides or "insectatachykinins," such as Locusta tachykinin (Lom-TK) III, produced dose-dependent calcium responses in stably transfected S2-STKR cells. Vertebrate tachykinins (or neurokinins) did not evoke any effect at concentrations up to 10(-5) M, but an antagonist of mammalian neurokinin receptors, spantide II, inhibited the Lom-TK III-induced calcium response. Further analysis showed that the agonist-induced intracellular release of calcium ions was not affected by pretreatment of the cells with pertussis toxin. The calcium rise was blocked by the phospholipase C inhibitor U73122. In addition, Lom-TK III was shown to have a stimulatory effect on the accumulation of both inositol 1,4,5-trisphosphate and cyclic AMP. These are the same second messengers that are induced in mammalian neurokinin-dependent signaling processes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence / genetics
  • Animals
  • Calcium / metabolism
  • Cell Line
  • Cyclic AMP / metabolism
  • Dose-Response Relationship, Drug
  • Drosophila melanogaster / cytology
  • Drosophila melanogaster / metabolism*
  • Humans
  • Infant, Newborn
  • Inositol 1,4,5-Trisphosphate / metabolism
  • Insect Proteins / pharmacology
  • Molecular Sequence Data
  • Muscidae / metabolism*
  • Peptide Fragments / agonists*
  • Receptors, Invertebrate Peptide / genetics
  • Receptors, Invertebrate Peptide / metabolism*
  • Receptors, Tachykinin / genetics
  • Receptors, Tachykinin / metabolism*
  • Tachykinins / agonists*
  • Tachykinins / pharmacology
  • Transfection


  • Insect Proteins
  • Peptide Fragments
  • Receptors, Invertebrate Peptide
  • Receptors, Tachykinin
  • STKR receptor, Stomoxys calcitrans
  • Tachykinins
  • locustatachykinin III protein, insect
  • Inositol 1,4,5-Trisphosphate
  • Cyclic AMP
  • Calcium

Associated data

  • GENBANK/U52347