ATPase switches controlling DNA replication initiation

Curr Opin Cell Biol. 2000 Jun;12(3):280-5. doi: 10.1016/s0955-0674(00)00089-2.


Proteins that bind and hydrolyze ATP are frequently involved in the early steps of DNA replication. Recent studies of Saccharomyces cerevisiae suggest that two members of the AAA+ ATPase family--the origin recognition complex and Cdc6p--have separable roles for ATP binding and ATP hydrolysis during eukaryotic DNA replication. Intriguingly, the proposed regulation of these eukaryotic replication proteins by ATP has functional similarities to the ATP-dependent control of the DnaA and DnaC initiation factors from Escherichia coli. Comparison of the ATP regulation of these factors suggests that ATP binding and hydrolysis acts as a molecular switch that couples key events during initiation of replication. This switch results in a significant change in protein function.

Publication types

  • Review

MeSH terms

  • Adenosine Triphosphatases / metabolism*
  • Adenosine Triphosphate / metabolism
  • Bacterial Proteins / metabolism
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism
  • DNA Helicases / metabolism
  • DNA Replication / physiology*
  • DNA-Binding Proteins / metabolism
  • Escherichia coli / metabolism
  • Escherichia coli Proteins*
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Mutation
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins*


  • Bacterial Proteins
  • CDC6 protein, S cerevisiae
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • DnaA protein, Bacteria
  • DnaC protein, E coli
  • Escherichia coli Proteins
  • Fungal Proteins
  • Saccharomyces cerevisiae Proteins
  • Adenosine Triphosphate
  • Adenosine Triphosphatases
  • DNA Helicases