The nuclear pore complex: a protein machine bridging the nucleus and cytoplasm

Curr Opin Cell Biol. 2000 Jun;12(3):361-71. doi: 10.1016/s0955-0674(00)00101-0.


Compositional analysis of nuclear pore complexes (NPCs) is nearing completion, and efforts are now focused on understanding how these protein machines work. Recent analysis of soluble transport factor interactions with NPC proteins reveals distinct and overlapping pathways for movement between the nucleus and cytoplasm. New fluorescence- and microscopy-based strategies have been used to monitor the pathway of NPC assembly and to reveal the dynamics of the NPC during transport.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Biological Transport, Active
  • Cell Nucleus / metabolism
  • Cytoplasm / metabolism
  • Humans
  • Mitosis
  • Nuclear Envelope / chemistry
  • Nuclear Envelope / metabolism*
  • Nuclear Proteins / metabolism
  • Protein Conformation
  • RNA, Messenger / metabolism
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / metabolism


  • Nuclear Proteins
  • RNA, Messenger