Vancomycin resistance in enterococci: reprogramming of the D-ala-D-Ala ligases in bacterial peptidoglycan biosynthesis

Chem Biol. 2000 May;7(5):R109-19. doi: 10.1016/s1074-5521(00)00116-2.


Vancomycin binds to bacterial cell-wall intermediates to achieve its antibiotic effect. Infections of vancomycin-resistant enterococci are, however, becoming an increasing problem; the bacteria are resistant because they synthesize different cell-wall intermediates. The enzymes involved in cell-wall biosynthesis, therefore, are potential targets for combating this resistance. Recent biochemical and crystallographic results are providing mechanistic and structural details about some of these targets.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Enterococcus / drug effects*
  • Enterococcus / enzymology*
  • Enterococcus / metabolism
  • Humans
  • Peptide Synthases / chemistry*
  • Peptide Synthases / metabolism*
  • Peptidoglycan / biosynthesis*
  • Vancomycin / pharmacology*
  • Vancomycin Resistance*


  • Peptidoglycan
  • Vancomycin
  • Peptide Synthases
  • D-alanylalanine synthetase