IL-12 induces SDS-stable class II alphabeta dimers in murine dendritic cells

Cytokine. 2000 Apr;12(4):401-4. doi: 10.1006/cyto.1999.0558.


We assessed the effect of rIL-12 on the expression of class II molecules and on the ratio between SDS-stable and unstable alphabeta dimers in dendritic cells. We found that in vitro exposure of the cells to IL-12 increased their surface expression of mature class II molecules, despite a marked decline in class II biosynthesis. This effect was accompanied by a striking increase in the overall proportion of SDS-stable heterodimers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western / methods
  • Dendritic Cells / drug effects
  • Dendritic Cells / immunology*
  • Dimerization
  • Histocompatibility Antigens Class II / biosynthesis*
  • Interleukin-12 / immunology*
  • Interleukin-12 / pharmacology
  • Mice
  • Mice, Inbred DBA
  • Sodium Dodecyl Sulfate*


  • Histocompatibility Antigens Class II
  • I-Ad antigen
  • Interleukin-12
  • Sodium Dodecyl Sulfate