HZwint-1, a novel human kinetochore component that interacts with HZW10

J Cell Sci. 2000 Jun:113 ( Pt 11):1939-50. doi: 10.1242/jcs.113.11.1939.


HZwint-1 (Human ZW10 interacting protein-1) was identified in a yeast two hybrid screen for proteins that interact with HZW10. HZwint-1 cDNA encodes a 43 kDa protein predicted to contain an extended coiled-coil domain. Immunofluorescence studies with sera raised against HZwint-1 protein revealed strong kinetochore staining in nocodazole-arrested chromosome spreads. This signal co-localizes at the kinetochore with HZW10, at a position slightly outside of the central part of the centromere as revealed by staining with a CREST serum. The kinetochore localization of HZwint-1 has been confirmed by following GFP fluorescence in HeLa cells transiently transfected with a plasmid encoding a GFP/HZwint-1 fusion protein. In cycling HeLa cells, HZwint-1 localizes to the kinetochore of prophase HeLa cells prior to HZW10 localization, and remains at the kinetochore until late in anaphase. This localization pattern, combined with the two-hybrid results, suggests that HZwint-1 may play a role in targeting HZW10 to the kinetochore at prometaphase. HZwint-1 was also found to localize to neocentromeres and to the active centromere of dicentric chromosomes. HZwint-1 thus appears to associate with all active centromeres, implying that it plays an important role in correct centromere function.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Biomarkers
  • Cell Cycle Proteins*
  • Chromosomal Proteins, Non-Histone / genetics
  • Chromosomal Proteins, Non-Histone / metabolism
  • Drosophila Proteins*
  • Genetic Testing
  • HeLa Cells
  • Humans
  • Insect Proteins / metabolism*
  • Intracellular Signaling Peptides and Proteins
  • Kinetochores / metabolism*
  • Molecular Sequence Data
  • Nuclear Proteins
  • Two-Hybrid System Techniques


  • Biomarkers
  • Cell Cycle Proteins
  • Chromosomal Proteins, Non-Histone
  • Drosophila Proteins
  • Insect Proteins
  • Intracellular Signaling Peptides and Proteins
  • Nuclear Proteins
  • ZWINT protein, human
  • Zw10 protein, Drosophila