Vagotomy does not inhibit high dose lipopolysaccharide-induced interleukin-1beta immunoreactivity in rat brain and pituitary gland

Neurosci Lett. 2000 May 19;285(3):169-72. doi: 10.1016/s0304-3940(00)01031-4.


In the present study, we examined whether the vagus nerve is involved in mediating lipopolysaccharide (LPS)-induced appearance of IL-1beta immunoreactive cells in the brain and pituitary gland. Rats were either sham-operated or subjected to subdiaphragmatic vagotomy. Four weeks later, pyrogen free saline or 400 microg/kg LPS was administered to the rats intraperitoneally. Four and 8 h later, the animals were intracardially perfused with 4% paraformaldehyde and tissues were prepared for IL-1beta immunocytochemistry. IL-1beta positive cells were observed at both time-intervals after LPS administration in the choroid plexus, meninges, circumventricular organs and pituitary gland of both sham-operated and vagotomized rats. We conclude that under the conditions studied, the vagus nerve does not mediate LPS-induced appearance of IL-1beta in the rat brain and pituitary gland.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Brain / drug effects
  • Brain / metabolism*
  • Interleukin-1 / metabolism*
  • Lipopolysaccharides / pharmacology
  • Male
  • Pituitary Gland / drug effects
  • Pituitary Gland / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Vagotomy
  • Vagus Nerve / drug effects
  • Vagus Nerve / physiology*


  • Interleukin-1
  • Lipopolysaccharides