The CUP1 promoter of Saccharomyces cerevisiae is inducible by copper in Pichia pastoris

Yeast. 2000 May;16(7):651-6. doi: 10.1002/(SICI)1097-0061(200005)16:7<651::AID-YEA580>3.0.CO;2-F.

Abstract

We report the construction of a Pichia pastoris integrating vector which contains the inducible CUP1 promoter from Saccharomyces cerevisiae. We show that the promoter is indeed inducible by copper when used in P. pastoris and that the level of induction is dependent on the amount of copper in the medium.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Copper / pharmacology*
  • Culture Media
  • Fungal Proteins*
  • Gene Expression Regulation, Fungal
  • Genetic Vectors / genetics
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Membrane Transport Proteins*
  • Metallothionein / genetics*
  • Metallothionein / metabolism
  • Peroxins
  • Pichia / genetics*
  • Pichia / growth & development
  • Pichia / metabolism
  • Plasmids / genetics
  • Promoter Regions, Genetic*
  • Receptors, Cytoplasmic and Nuclear / genetics
  • Receptors, Cytoplasmic and Nuclear / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Ubiquitin-Protein Ligases
  • beta-Galactosidase / metabolism

Substances

  • CUP1-1 protein, S cerevisiae
  • Carrier Proteins
  • Culture Media
  • Fungal Proteins
  • Membrane Proteins
  • Membrane Transport Proteins
  • PEX10 protein, S cerevisiae
  • Peroxins
  • Pex22 protein, Pichia pastoris
  • Receptors, Cytoplasmic and Nuclear
  • Saccharomyces cerevisiae Proteins
  • copper thionein
  • Copper
  • Metallothionein
  • Ubiquitin-Protein Ligases
  • beta-Galactosidase