Delayed graft rejection in pre-vascularised corneas after subconjunctival injection of clodronate liposomes

Curr Eye Res. 2000 Apr;20(4):322-4.


Purpose: To test the effects of clodronate liposomes on graft survival and neovascularisation after transplantation in pre-vascularised recipient corneas.

Methods: Corneal neovascularisation was induced in F344 rats by injecting heat inactivated rabbit serum intrastromally. After 4 weeks F344 rats were orthotopically grafted with corneal buttons from DA rats. Directly after transplantation and on 2, 4, 6 and 8 days postoperatively clodronate liposomes were administrated subconjunctivally in one group, whereas the other group remained untreated. For 60 days grafts were observed for signs of graft rejection and neovascularisation.

Results: Graft survival was significantly prolonged, but not prevented in clodronate liposome treated rats compared to untreated rats ( p =.004). Also clodronate liposome administration delays growth of corneal neovascularisation after transplantation.

Conclusions: Previous studies revealed that clodronate liposomes prevent corneal graft rejection and reduce neovascularisation in orthotopic corneal allotransplantation in rats. This study shows that also in pre-vascularised recipient corneas subconjunctival administration of clodronate liposomes seems to delay corneal graft rejection and reduces neovascularisation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Clodronic Acid / administration & dosage*
  • Clodronic Acid / therapeutic use
  • Conjunctiva
  • Cornea / blood supply*
  • Corneal Transplantation*
  • Drug Carriers
  • Graft Rejection / prevention & control*
  • Injections
  • Liposomes
  • Male
  • Neovascularization, Pathologic / etiology
  • Neovascularization, Pathologic / prevention & control
  • Rabbits / blood
  • Rats
  • Rats, Inbred F344
  • Rats, Inbred Strains
  • Time Factors


  • Drug Carriers
  • Liposomes
  • Clodronic Acid