PDGF-C Is a New Protease-Activated Ligand for the PDGF Alpha-Receptor

Nat Cell Biol. 2000 May;2(5):302-9. doi: 10.1038/35010579.

Abstract

Platelet-derived growth factors (PDGFs) are important in many types of mesenchymal cell. Here we identify a new PDGF, PDGF-C, which binds to and activates the PDGF alpha-receptor. PDGF-C is activated by proteolysis and induces proliferation of fibroblasts when overexpressed in transgenic mice. In situ hybridization analysis in the murine embryonic kidney shows preferential expression of PDGF-C messenger RNA in the metanephric mesenchyme during epithelial conversion. Analysis of kidneys lacking the PDGF alpha-receptor shows selective loss of mesenchymal cells adjacent to sites of expression of PDGF-C mRNA; this is not found in kidneys from animals lacking PDGF-A or both PDGF-A and PDGF-B, indicating that PDGF-C may have a unique function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • COS Cells
  • Endopeptidases / metabolism*
  • Epithelial Cells / chemistry
  • Epithelial Cells / enzymology
  • Gene Expression / physiology
  • Gene Expression Regulation, Developmental
  • Humans
  • In Situ Hybridization
  • Insecta
  • Kidney / chemistry
  • Kidney / embryology
  • Kidney / enzymology
  • Ligands
  • Lymphokines
  • Mesoderm / chemistry
  • Mesoderm / enzymology
  • Mice
  • Molecular Sequence Data
  • Myocardium / chemistry
  • Myocardium / enzymology
  • Platelet-Derived Growth Factor / chemistry
  • Platelet-Derived Growth Factor / genetics*
  • Platelet-Derived Growth Factor / metabolism*
  • Platelet-Derived Growth Factor / pharmacology
  • Protein Structure, Tertiary
  • RNA, Messenger / analysis
  • Rabbits
  • Receptor, Platelet-Derived Growth Factor alpha / metabolism*
  • Sequence Homology, Amino Acid
  • Transgenes / physiology

Substances

  • Ligands
  • Lymphokines
  • Platelet-Derived Growth Factor
  • RNA, Messenger
  • platelet-derived growth factor A
  • platelet-derived growth factor C
  • Receptor, Platelet-Derived Growth Factor alpha
  • Endopeptidases

Associated data

  • GENBANK/AF244813