Organization and expression of two Arabidopsis DREB2 genes encoding DRE-binding proteins involved in dehydration- and high-salinity-responsive gene expression

Plant Mol Biol. 2000 Mar;42(4):657-65. doi: 10.1023/a:1006321900483.

Abstract

In plants, a cis-acting element, DRE/CRT, is involved in ABA-independent gene expression in response to dehydration and low-temperature stress. To understand signal transduction pathways from perception of the dehydration stress signal to gene expression, we characterized a gene family for DRE/CRT-binding proteins DREB2A and DREB2B in Arabidopsis thaliana. Northern analysis showed that both genes are induced by dehydration and high-salt stress. Organ-specific northern analysis with gene-specific probes showed that these genes are strongly induced in roots by high-salt stress and in stems and roots by dehydration stress. The DREB2A gene is located on chromosome 5, and DREB2B on chromosome 3. We screened an Arabidopsis genomic DNA library with cDNA fragments of DREB2A and DREB2B as probes, and isolated DNA fragments that contained 5'-flanking regions of these genes. Sequence analysis showed that both genes are interrupted by a single intron at identical positions in their leader sequence. Several conserved sequences were found in the promoter regions of both genes. The beta-glucuronidase (GUS) reporter gene driven by the DREB2 promoters was induced by dehydration and high-salt stress in transgenic Arabidopsis plants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / drug effects
  • Arabidopsis / genetics*
  • Arabidopsis Proteins*
  • Base Sequence
  • Chromosome Mapping
  • DNA, Plant / chemistry
  • DNA, Plant / genetics
  • DNA, Plant / isolation & purification
  • DNA-Binding Proteins / genetics*
  • Gene Expression
  • Gene Expression Regulation, Plant / drug effects
  • Genes, Plant / genetics
  • Glucuronidase / drug effects
  • Glucuronidase / genetics
  • Glucuronidase / metabolism
  • Introns
  • Molecular Sequence Data
  • Plant Proteins / genetics
  • Plants, Genetically Modified
  • Promoter Regions, Genetic / genetics
  • Protein Isoforms / genetics
  • RNA, Messenger / drug effects
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Recombinant Fusion Proteins / drug effects
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Sodium Chloride / pharmacology*
  • Tissue Distribution
  • Transcription Factors / genetics*
  • Water / pharmacology*

Substances

  • Arabidopsis Proteins
  • DNA, Plant
  • DNA-Binding Proteins
  • DREB2A protein, Arabidopsis
  • Plant Proteins
  • Protein Isoforms
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • Transcription Factors
  • Water
  • Sodium Chloride
  • Glucuronidase

Associated data

  • GENBANK/AB016570
  • GENBANK/AB016571