Identification of a small RNA within the pdh gene cluster of Mycoplasma pneumoniae and Mycoplasma genitalium

J Bacteriol. 2000 Jun;182(11):3281-4. doi: 10.1128/JB.182.11.3281-3284.2000.


A highly abundant and heterogeneous small RNA about 205 to 210 bases long named MP200 RNA has been identified in Mycoplasma pneumoniae. It was localized on the genome within a 319-bp-long intergenic space of the pyruvate dehydrogenase (pdh) gene cluster. A database search at the DNA level revealed the highest similarity to a sequence located within the pdh gene cluster of Mycoplasma genitalium that was also shown to be transcribed into two abundant, but smaller RNAs than the ones in Mycoplasma pneumoniae. The RNAs from both M. pneumoniae and M. genitalium have the potential to code for cysteine-rich 29- and 23-amino-acid-long peptides, but so far, these peptides have not been identified experimentally in bacterial protein extracts.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Molecular Sequence Data
  • Multigene Family*
  • Mycoplasma / enzymology
  • Mycoplasma / genetics*
  • Mycoplasma pneumoniae / enzymology
  • Mycoplasma pneumoniae / genetics*
  • Nucleic Acid Conformation
  • Pyruvate Dehydrogenase Complex / genetics*
  • RNA, Bacterial / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Species Specificity


  • MP200 RNA
  • Pyruvate Dehydrogenase Complex
  • RNA, Bacterial