Amniotic membrane transplantation for acute chemical or thermal burns

Ophthalmology. 2000 May;107(5):980-9; discussion 990. doi: 10.1016/s0161-6420(00)00024-5.


Purpose: To determine whether preserved human amniotic membrane (AM) can be used to treat ocular burns in the acute stage.

Design: Prospective, noncomparative, interventional case series.

Participants: Thirteen eyes from 11 patients with acute burns, 10 eyes with chemical burns and 3 with thermal burns of grades II-III (7 eyes) and grade IV (6 eyes), treated at 7 different facilities.

Methods: Patients received amniotic membrane transplantation (AMT) within 2 weeks after the injury.

Main outcome measures: Integrity of ocular surface epithelium and visual acuity during 9 months of follow-up.

Results: Ten patients were male and one patient was female; most were young (38.2 +/- 10.6 years). For a follow-up of 8.8 + 4.7 months, 11 of 13 eyes (84.63%) showed epithelialization within 2 to 5 weeks (23.7 +/- 9.8 days), and final visual acuity improved > or = 6 lines (6 eyes), 4 to 5 lines (2 eyes), and 1 to 3 lines (2 eyes); only one eye experienced a symblepharon. Eyes with burns of grade II to III showed more visual improvement (7.3 +/- 3 lines) than those with burns of grade IV (2.3 +/- 3.0 lines; P < 0.05, unpaired t test). In the group with grade II or III burns, none had limbal stem cell deficiency. All eyes in the group with grade IV burns did experience limbal stem cell deficiency.

Conclusions: Amniotic membrane transplantation is effective in promoting re-epithelialization and reducing inflammation, thus preventing scarring sequelae in the late stage. In mild to moderate burns, AMT alone rapidly restores both corneal and conjunctival surfaces. In severe burns, however, it restores the conjunctival ocular surface without debilitating symblepharon and reduces limbal stromal inflammation, but does not prevent limbal stem cell deficiency, which requires further limbal stem cell transplantation. These results underscore the importance of immediate intervention in the acute stage of eyes with severely damaged ocular surface. Further prospective randomized studies including a control group are required to determine the effectiveness of AMT in acute chemical and thermal burns of the eye.

Publication types

  • Case Reports
  • Clinical Trial
  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acids
  • Acute Disease
  • Adult
  • Alkalies
  • Amnion / transplantation*
  • Burns / surgery
  • Burns, Chemical / classification
  • Burns, Chemical / surgery*
  • Epithelium, Corneal / cytology
  • Epithelium, Corneal / physiology
  • Eye Burns / chemically induced*
  • Eye Burns / classification
  • Eye Burns / surgery
  • Female
  • Humans
  • Male
  • Middle Aged
  • Prospective Studies
  • Stem Cells / cytology
  • Stem Cells / physiology
  • Tissue Preservation
  • Visual Acuity


  • Acids
  • Alkalies