Efficient gene transfer into primary human CD8+ T lymphocytes by MuLV-10A1 retrovirus pseudotype

Hum Gene Ther. 2000 May 1;11(7):1005-14. doi: 10.1089/10430340050015310.


Efficient and stable gene transfer into primary human T lymphocytes would greatly improve their use for adoptive transfer to treat acquired disorders, viral diseases, and cancer. We have constructed retroviral vector pseudotypes of amphotropic murine leukemia viruses (A-MuLV, MuLV-10A1), gibbon ape leukemia virus (GaLV), and feline endogenous virus (RD114) containing the enhanced green fluorescent protein (GFP) as a marker gene. Transduction of primary human CD8+ T lymphocytes by the different GFP-retrovirus pseudotypes revealed the superiority of MuLV-10A1 in comparison with A-MuLV, GaLV, and RD114, respectively. The superior transduction efficacy of CD8+ T cells by MuLV-10A1 correlates with a longer half-life of this pseudotype in comparison with A-MuLV and, as shown by interference analysis with the human T cell line HUT78, by the utilization of both the A-MuLV receptor (Pit2) and the GaLV receptor (Pit1) for cell entry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CD8-Positive T-Lymphocytes / physiology*
  • CD8-Positive T-Lymphocytes / virology
  • Gene Transfer Techniques*
  • Genetic Vectors*
  • Green Fluorescent Proteins
  • Humans
  • Leukemia Virus, Murine / genetics*
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Receptors, Virus / genetics
  • Receptors, Virus / metabolism
  • Retroviridae / genetics
  • Transduction, Genetic


  • Luminescent Proteins
  • Receptors, Virus
  • leukemia virus receptor, gibbon ape
  • Green Fluorescent Proteins