Sequencing of peach latent mosaic viroid variants from nine North American peach cultivars shows that this RNA folds into a complex secondary structure

M Pelchat; D Lévesque; J Ouellet; S Laurendeau; S Lévesque; J Lehoux; D A Thompson; K C Eastwell; L J Skrzeczkowski; J P Perreault

doi:10.1006/viro.2000.0288

Sequencing of peach latent mosaic viroid variants from nine North American peach cultivars shows that this RNA folds into a complex secondary structure

Virology. 2000 May 25;271(1):37-45. doi: 10.1006/viro.2000.0288.

Authors

M Pelchat¹, D Lévesque, J Ouellet, S Laurendeau, S Lévesque, J Lehoux, D A Thompson, K C Eastwell, L J Skrzeczkowski, J P Perreault

Affiliation

¹ Département de Biochimie, Université de Sherbrooke, Sherbrooke, Québec, J1H 5N4, Canada.

PMID: 10814568
DOI: 10.1006/viro.2000.0288

Abstract

We sequenced 34 new peach latent mosaic viroid (PLMVd) variants isolated from nine different peach cultivars. This study provides the widest view of PLMVd diversity reported to date and includes the original characterization of North American variants, which cannot be differentiated from European sequences. PLMVd appears as a species in which each isolate is a complex mixture of RNAs. Analysis of base-pair covariations supports the hypothesis that PLMVd folds into a complex branched structure with the potential of including three new pseudoknots. The resulting "globular-like" structure is in contrast to the rod-like one adopted by most other viroids.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Cloning, Molecular
Consensus Sequence
Molecular Sequence Data
Mosaic Viruses / genetics*
Nucleic Acid Conformation*
RNA, Viral / chemistry*
Rosales / virology*
Sequence Analysis

Substances

RNA, Viral