Identification of complement-receptor lymphocytes (B cells) in lymph nodes and tumor infiltrates

J Urol. 1975 Dec;114(6):915-21. doi: 10.1016/s0022-5347(17)67174-x.


To monitor the distribution of lymphocyte sub-populations infiltrating tumors, a simplified modification of standard techniques was developed to identify the complement-receptor B lymphocytes in frozen tissue sections. This technique is based on binding of antibody-coated bovine erythrocytes by B lymphocytes in the presence of sub-hemolytic concentrations of mouse complement. B lymphocytes can be identified in frozen tissue sections by adherent sensitized bovine erythrocytes. The specificity of the technique for B lymphocytes was established by 1) absence of spontaneous rosette formation with bovine erythrocytes by human T lymphocytes, 2) specific binding of bovine erythrocyte-antibody-complement complexes to B cell regions of normal lymph nodes and spleen and 3) binding of erythrocyte-antibody-complement to a high proportion of chronic lymphocytic leukemia (a B cell leukemia) lymphocytes. Five transitional carcinomas studied had regions of mononuclear infiltration that were virtually devoid of complement-receptor lymphocytes. The results suggest that lymphocytes infiltrating bladder carcinomas may be predominantly T lymphocytes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • B-Lymphocytes / immunology*
  • Binding Sites
  • Binding Sites, Antibody
  • Carcinoma, Transitional Cell / immunology*
  • Cattle
  • Complement C3*
  • Complement System Proteins*
  • Erythrocytes / immunology
  • Humans
  • Immune Adherence Reaction
  • Leukemia, Lymphoid / immunology*
  • Lymph Nodes / immunology*
  • Prognosis
  • Spleen / immunology
  • Urinary Bladder Neoplasms / immunology*


  • Complement C3
  • Complement System Proteins