To evaluate the effects of enterectomy on cellular and biochemical parameters of blood and peritoneal fluid, an experiment was conducted using 10 Iranian crossbred male goats. Ten milliliter of blood and 1-1.5ml of peritoneal fluid were sampled from all animals prior to operation for the estimation of control values. Enterectomy was performed under local anesthesia. Blood and peritoneal fluid samples were collected at 24, 48, 72 and 96h after enterectomy. The results revealed that after enterectomy, the number of WBCs, neutrophils, monocytes and band neutrophils in the blood significantly increased (p<0.05). However, the percent of lymphocytes decreased significantly (p<0.05). The concentrations of glucose and blood urea nitrogen (BUN) significantly increased (p<0.05). Furthermore, following the operation, the number of WBCs and the percent of neutrophils and band neutrophils in the peritoneal fluid significantly increased (p<0.05). In contrast, the percent of lymphocytes and monocytes in the peritoneal fluid decreased significantly (p<0.05). The concentrations of protein and the activities of amylase and alkaline phosphatase (ALP) in the peritoneal fluid increased significantly (p<0.05). However, the concentration of glucose decreased significantly (p<0.05). This study showed that enterectomy can have profound effects on blood and peritoneal fluid parameters.