Rapid determination of membrane transport parameters in adherent cells

Biotechniques. 2000 May;28(5):926-8, 930, 932. doi: 10.2144/00285st06.

Abstract

Reported here is a new method that permits rapid (approximately 5 s) determinations of membrane transport phenomena in cells grown in monolayers at the base of 17-mm glass scintillation vials. The method is convenient, cost effective and requires no special apparatus. Initial uptake rates, steady-state and free substrate levels are demonstrated in ZR-75-1 breast cancer and Chinese hamster ovary cell lines using methotrexate, a model agent transported by the reduced folate carrier. The technique should be applicable to the study of the transport properties in a broad range of substrates and cells in monolayer culture.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Technical Report

MeSH terms

  • Animals
  • Breast Neoplasms
  • CHO Cells
  • Cell Membrane / metabolism
  • Cell Membrane Permeability / physiology
  • Cricetinae
  • Drug Design
  • Drug Industry / methods*
  • Folic Acid Antagonists / pharmacokinetics*
  • Humans
  • Methotrexate / pharmacokinetics*
  • Scintillation Counting
  • Transfection
  • Tritium
  • Tumor Cells, Cultured

Substances

  • Folic Acid Antagonists
  • Tritium
  • Methotrexate