Identification and cataloging of genes induced by long-lasting long-term potentiation in awake rats

J Neurochem. 2000 Jun;74(6):2239-49. doi: 10.1046/j.1471-4159.2000.0742239.x.

Abstract

Maintenance of long-term potentiation (LTP) requires de novo gene expression. Here we report the direct isolation, using PCR-differential display, of genes whose expression level was altered after induction of long-lasting LTP in the hippocampus of freely moving awake rats. Differential display using 480 primer combinations revealed 17 cDNA bands that showed a reproducible change in expression level. These cDNAs represented at least 10 different genes (termed RM1-10), all of which showed up-regulation at 75 min after LTP induction and a return to basal expression levels within 24 h. Three of these genes were known only from expressed sequence tags (RM1-3), two were known genes whose up-regulation by LTP has not been described (GADD153/CHOP and ler5), and five were known genes whose up-regulation by LTP has already been reported (MAPK phosphatase, NGFI-A/zif268, vesl-1S/homer-1a, Ag2, and krox-20). We characterized the expression profiles of genes in the two former categories with respect to NMDA receptor dependency, tissue specificity, and developmental regulation using northern blotting and semiquantitative RT-PCR. The up-regulation of all five of these genes was NMDA receptor-dependent and correlated with the persistence of LTP, suggesting that these genes may play functional roles in prolonged LTP maintenance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Brain Chemistry / genetics*
  • Cloning, Molecular
  • DNA Primers
  • DNA, Complementary
  • Electrodes, Implanted
  • Gene Expression Regulation, Developmental
  • Genetic Testing / methods
  • Long-Term Potentiation / genetics*
  • Male
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics
  • Neuronal Plasticity / genetics
  • Polymerase Chain Reaction / methods
  • RNA, Messenger / analysis
  • Rats
  • Rats, Wistar
  • Receptors, N-Methyl-D-Aspartate / genetics*
  • Viscera / chemistry
  • Wakefulness / genetics*

Substances

  • DNA Primers
  • DNA, Complementary
  • Nerve Tissue Proteins
  • RNA, Messenger
  • Receptors, N-Methyl-D-Aspartate

Associated data

  • GENBANK/AB032083
  • GENBANK/AB032084
  • GENBANK/AB032085
  • GENBANK/AB032086
  • GENBANK/AB032087