The effect of 6-methylthiohexyl isothiocyanate isolated from Wasabia japonica (wasabi) on 4-(methylnitrosamino)-1-(3-pyridyl)-1-buatnone-induced lung tumorigenesis in mice

Cancer Lett. 2000 Jul 31;155(2):115-20. doi: 10.1016/s0304-3835(00)00364-5.


The present study was undertaken to estimate the effect of 6-methylthiohexyl isothiocyanate (6MHITC) isolated from Wasabia japonica (wasabi) pretreatment on 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone(NNK)-induced lung tumorigenesis in mice. Pretreatment with 6MHITC for 4 consecutive days at a daily dose of 5 micromol significantly inhibited NNK-induced O(6)-methylguanine formation in lungs at 4 h after the injection. In conjugation with this inhibitory effect, 6MHITC suppressed the increase in proliferating nuclear cell antigen level as well as ornithine decarboxylase activity at a promotion stage of NNK-induced lung tumorigenesis. Finally, this treatment of 6MHITC suppressed the NNK-induced lung tumorigenesis in mice. These results suggest that 6MHITC inhibits the development of lung tumors in mice treated with NNK, due to the suppression of initiation stage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Weight / drug effects
  • Carcinogens*
  • Female
  • Guanine / analogs & derivatives
  • Guanine / metabolism
  • Immunoblotting
  • Isothiocyanates / pharmacology*
  • Lung Neoplasms / chemically induced*
  • Lung Neoplasms / prevention & control
  • Mice
  • Nitrosamines*
  • Ornithine Decarboxylase / metabolism
  • Plant Extracts / pharmacology*
  • Proliferating Cell Nuclear Antigen / biosynthesis
  • Spices


  • Carcinogens
  • Isothiocyanates
  • Nitrosamines
  • Plant Extracts
  • Proliferating Cell Nuclear Antigen
  • isothiocyanic acid
  • Guanine
  • 6-methylthiohexyl isothiocyanate
  • 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone
  • O-(6)-methylguanine
  • Ornithine Decarboxylase