Mitochondrial DNA analysis of Exophiala jeanselmei var. lecanii-corni and Exophiala castellanii

Mycopathologia. 1999;146(2):75-7. doi: 10.1023/a:1007057713782.

Abstract

A Japanese clinical isolate (KU-A-0094) which was identified by de Hoog et al. as Exophiala jeanselmei var. lecanii-corni with difficulty, was compared with 5 strains including the type cultures of E. jeanselmei var. lecanii-corni, var. jeanselmei and E. castellanii using RFLP (restriction fragment length polymorphism) patterns of mtDNA (mitochondrial DNA). RFLP patterns of KUA-0094 were identical with those of E. jeanselmei var. lecanii-corni and different from those of E. castellanii with restriction enzymes of HaeIII, MspI and hindIII. Therefore, de Hoog et al.'s identification of KU-A-0094 was confirmed. Additionally, mtDNA-RFLP patterns of E. jeanselmei var. lecanii-corni and E. jeanselmei var. jeanselmei were also different from each other. Consequently E. jeanselmei var. lecanii-corni seem to be a species in its own right rather than a variant of E. jeanselmei.

MeSH terms

  • DNA, Fungal / analysis
  • DNA, Mitochondrial / analysis*
  • Exophiala / classification*
  • Exophiala / genetics*
  • Exophiala / isolation & purification
  • Humans
  • Mycoses / microbiology
  • Polymorphism, Restriction Fragment Length

Substances

  • DNA, Fungal
  • DNA, Mitochondrial