In vivo electron spin resonance assessment of decay constant of nitroxyl radical in selenium-deficient rat

Biol Pharm Bull. 2000 May;23(5):641-4. doi: 10.1248/bpb.23.641.


In vivo reducing capacity and cytosolic glutathione peroxidase (GSH-Px) activity in liver homogenate were evaluated in 6 weeks old Se-deficient and normal rats. GSH-Px was significantly lower in Se-deficient rats than in normal rats. In vivo reducing capacity in head and liver parts, estimated from in vivo signal decay of a nitroxyl spin probe using a low frequency (300 MHz) ESR spectrometer, was significantly decreased in Se-deficient rats, suggesting a decrease of antioxidant capacity in Se-deficient rats.

MeSH terms

  • Animals
  • Electron Spin Resonance Spectroscopy / methods*
  • Female
  • Glutathione Peroxidase / metabolism
  • Liver / enzymology
  • Liver / metabolism
  • Male
  • Nitrogen Oxides / chemistry*
  • Nitrogen Oxides / metabolism
  • Oxidative Stress
  • Rats
  • Rats, Wistar
  • Selenium / deficiency*
  • Selenium / metabolism


  • Nitrogen Oxides
  • Glutathione Peroxidase
  • nitroxyl
  • Selenium