Cytotoxic T lymphocyte (CTL) responses to human cytomegalovirus pp65, IE1-Exon4, gB, pp150, and pp28 in healthy individuals: reevaluation of prevalence of IE1-specific CTLs

J Infect Dis. 2000 May;181(5):1537-46. doi: 10.1086/315445. Epub 2000 May 15.

Abstract

The prevalence of human cytomegalovirus (HCMV) pp65-, pp150-, IE1-exon4-, gB- and pp28-specific cytotoxic T lymphocyte (CTL) responses was compared among 34 healthy individuals, grouped by neutralizing antibody titers. Moderately and highly seropositive donors showed predominantly pp65- and IE1-exon4-specific CTL responses (92% and 76% of the donors, respectively), with similar precursor frequencies in the 2 donors tested. In addition, highly seropositive and a few moderately seropositive donors showed CTL responses to gB and pp150 (33% and 30% of the donors, respectively). No individual recognized pp28 as a target in the CTL assay. Phenotypic analysis revealed a mixed effector population of CD4+ and CD8+ (1 donor) or only CD8+ cells for pp65-specific effectors (2 donors). IE1-exon4- and pp150-specific effectors were CD8+ (2 donors and 1 donor, respectively), whereas gB-specific CTLs were CD4+ (1 donor). These data may help to design a cellular immunity-based vaccine effective against HCMV diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antigens, Viral / immunology*
  • CD8-Positive T-Lymphocytes / immunology
  • Continental Population Groups
  • Cytomegalovirus / genetics
  • Cytomegalovirus / immunology*
  • Exons
  • Humans
  • Immediate-Early Proteins / genetics
  • Immediate-Early Proteins / immunology*
  • Middle Aged
  • Phosphoproteins / immunology*
  • Reference Values
  • T-Lymphocytes, Cytotoxic / immunology*
  • T-Lymphocytes, Cytotoxic / virology*
  • Viral Envelope Proteins / immunology*
  • Viral Matrix Proteins / immunology*
  • Viral Proteins / immunology*

Substances

  • Antigens, Viral
  • IE1 protein, cytomegalovirus
  • Immediate-Early Proteins
  • Phosphoproteins
  • Viral Envelope Proteins
  • Viral Matrix Proteins
  • Viral Proteins
  • cytomegalovirus matrix protein 65kDa
  • glycoprotein B, Simplexvirus
  • human cytomegalovirus protein pp28
  • pp150 protein, Cytomegalovirus