Inhibitory effect of resveratrol on free radical generation in blood platelets

Acta Biochim Pol. 1999;46(4):961-6.

Abstract

Resveratrol (3,4',5-trihydroxystilbene), a compound found in many plants, has been shown to prevent coronary heart diseases and to exert a variety of antiinflammatory and anticancerogenic effects. It is effective in lowering the level of serum lipids and in inhibiting platelet aggregation. We evaluated the effect of trans-resveratrol on the production of free radicals in pig blood platelets and showed that resveratrol inhibited the production of different reactive oxygen species (O2*-, H2O2, singlet oxygen and organic radicals) measured by the luminol-dependent chemiluminescence in resting platelets (P < 0.05). Resveratrol inhibited also the generation of radicals in platelets activated by thrombin (P < 0.05). Treatment of platelets with resveratrol at concentrations of 6.25 and 12.5 microg/ml caused a statistically insignificant increase in the production of O2*- in these cells, as measured by reduction of cytochrome c; however, at higher doses (25, 50 and 100 microg/ml) resveratrol distinctly reduced the generation of O2*- in platelets (P < 0.05). We suggest that free radicals play an important role in the reduced reactivity of blood platelets induced by resveratrol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Blood Platelets / drug effects*
  • Blood Platelets / metabolism*
  • Free Radicals / blood
  • Humans
  • Hydrogen Peroxide / blood
  • In Vitro Techniques
  • Luminescent Measurements
  • Oxygen / blood
  • Reactive Oxygen Species / metabolism*
  • Resveratrol
  • Singlet Oxygen
  • Stilbenes / pharmacology*
  • Superoxides / blood
  • Swine
  • Thrombin / pharmacology

Substances

  • Antioxidants
  • Free Radicals
  • Reactive Oxygen Species
  • Stilbenes
  • Superoxides
  • Singlet Oxygen
  • Hydrogen Peroxide
  • Thrombin
  • Resveratrol
  • Oxygen