Repression of Acinetobacter vanillate demethylase synthesis by VanR, a member of the GntR family of transcriptional regulators

FEMS Microbiol Lett. 2000 Jun 1;187(1):65-8. doi: 10.1111/j.1574-6968.2000.tb09138.x.


Vanillate is converted to protocatechuate by the action of vanillate demethylase encoded by vanAB. Convergent upon and overlapping Acinetobacter vanB is an open reading frame encoding a member of the gntR repressor family and designated vanR. This gene organization differs from that found in a Pseudomonas isolate. An Acinetobacter strain with a knockout mutation in vanR constitutively converted vanillate to protocatechuate. Reverse transcriptase-polymerase chain reaction was used to demonstrate that control of vanAB was exerted at the level of transcription.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acinetobacter / enzymology
  • Acinetobacter / genetics*
  • Bacterial Proteins*
  • Down-Regulation
  • Oxidoreductases, O-Demethylating / biosynthesis*
  • Oxidoreductases, O-Demethylating / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription Factors / genetics*
  • Transcription, Genetic


  • Bacterial Proteins
  • Transcription Factors
  • VanR protein, bacteria
  • Oxidoreductases, O-Demethylating
  • vanillate O-demethylase