Chemoprevention of 2-acetylaminofluorene-induced hepatotoxicity and lipid peroxidation in rats by kolaviron--a Garcinia kola seed extract

Food Chem Toxicol. 2000 Jun;38(6):535-41. doi: 10.1016/s0278-6915(00)00039-9.

Abstract

The effect of kolaviron, a mixture of Garcinia biflavonoid 1 (GB1), Garcinia biflavonoid 2 (GB2) and kolaflavanone, used in the treatment of various ailments in southern Nigeria on hepatotoxicity and lipid peroxidation induced by 2-acetylaminofluorene (2-AAF) in rats was investigated. The ability of butylated hydroxyanisole (BHA) to attenuate the toxic effect of 2-AAF was also examined. Kolaviron administered orally to rats at a dose of 100mg/kg body weight twice a day for 1 week before challenge with 2-AAF (200mg/kg feed) and continuously for 3 weeks at a single dose of 200mg/kg body weight reversed the 2-AAF-mediated decrease in final body weight and relative organ weights, especially the liver. BHA was administered at a dose of 7.5g/kg feed to the animals for 4 weeks. The extract decreased significantly the 2-AAF-mediated increase in the activity of aspartate aminotransferase, alanine aminotransferase, gamma-glutamyl transferase and ornithine carbamyl transferase by 58%, 62%, 60% and 67%, respectively. BHA elicited respectively 55%, 63%, 57% and 65% reduction in the 2-AAF induced-increase in the activities of these enzymes. Histological examination of the liver slices correlated with the changes in serum enzyme alterations. Similarly, kolaviron decreased the 2-AAF reduction of 5'-nucleotidase and glucose-6-phosphatase activities by 63% and 60%, respectively while BHA elicited 59% and 61% decrease in the activities of these enzymes. Simultaneous administration of kolaviron with 2-AAF inhibited microsomal lipid peroxidation as assessed by the thiobarbituric acid reacting substances (TBARS) formation by 66%. BHA produced a 64% reduction in TBARS formation. In the present study, kolaviron appears to act as an in vivo natural antioxidant and an effective hepatoprotective agent and is as effective as BHA.

MeSH terms

  • 2-Acetylaminofluorene / antagonists & inhibitors
  • 2-Acetylaminofluorene / toxicity*
  • Animals
  • Anticarcinogenic Agents / pharmacology*
  • Biflavonoids*
  • Carcinogens / toxicity*
  • Chemical and Drug Induced Liver Injury
  • Chemoprevention
  • Disease Models, Animal
  • Drug Interactions
  • Flavonoids / pharmacology*
  • Flavonoids / therapeutic use
  • Lipid Peroxidation / drug effects*
  • Liver Diseases / prevention & control*
  • Male
  • Protective Agents / pharmacology
  • Protective Agents / therapeutic use
  • Rats
  • Rats, Wistar
  • Seeds / chemistry

Substances

  • Anticarcinogenic Agents
  • Biflavonoids
  • Carcinogens
  • Flavonoids
  • Protective Agents
  • Garcinia biflavonoid 1
  • Garcinia biflavonoid 2
  • 2-Acetylaminofluorene
  • kolaviron