Cloning, expression, and sequence analysis of the gene encoding the alkali-stable, thermostable endoxylanase from alkalophilic, mesophilic Bacillus sp. Strain NG-27

Appl Environ Microbiol. 2000 Jun;66(6):2631-5. doi: 10.1128/AEM.66.6.2631-2635.2000.

Abstract

Alkalophilic Bacillus sp. strain NG-27 produces a 42-kDa endoxylanase active at 70 degrees C and at a pH of 8.4. The gene for this endoxylanase was cloned and sequenced. The gene contained one open reading frame of 1,215 bases. An active site characteristic of the family 10 beta-glycanases was recognized between amino acids 303 and 313, with the active glutamate at position 310. Though highly thermostable, the enzyme contains no cysteine residue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacillus / enzymology
  • Bacillus / genetics*
  • Base Sequence
  • Binding Sites
  • Cloning, Molecular
  • Endo-1,4-beta Xylanases
  • Enzyme Stability
  • Hydrogen-Ion Concentration
  • Molecular Sequence Data
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Temperature
  • Xylosidases / chemistry
  • Xylosidases / genetics*
  • Xylosidases / metabolism*

Substances

  • Xylosidases
  • Endo-1,4-beta Xylanases

Associated data

  • GENBANK/AF015445