Abstract
The effects of huperzine A (HupA), a novel acetylcholinesterase inhibitor, on Abeta(25-35)-induced cell lesion, level of lipid peroxidation, antioxidant enzyme activities were investigated in the rat pheochromocytoma line PC12. Following a 48 h exposure of the cells to Abeta(25-35), a significant reduction in cell survival and activities of glutathione peroxidase (GSH-Px) and catalase (CAT), as well as increased production of malondialdehyde (MDA) and superoxide dismutase (SOD) were observed. Preincubation of the cells with HupA prior to Abeta(25-35) exposure elevated the cell survival and GSH-Px and CAT activities, and decreased the level of MDA and SOD activity. The results indicate that HupA has protective effects against Abeta-induced cell toxicity, which might be beneficial for the treatment of Alzheimer's disease.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaloids
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Amyloid beta-Peptides / pharmacology*
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Animals
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Catalase / antagonists & inhibitors
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Catalase / metabolism
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Cell Survival / drug effects
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Cholinesterase Inhibitors / pharmacology*
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Glutathione Peroxidase / antagonists & inhibitors
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Glutathione Peroxidase / metabolism
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Malondialdehyde / metabolism
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Neuroprotective Agents / pharmacology*
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Oxidative Stress / physiology*
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PC12 Cells / drug effects*
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PC12 Cells / enzymology
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PC12 Cells / pathology*
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PC12 Cells / physiology
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Peptide Fragments / pharmacology*
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Rats
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Sesquiterpenes / pharmacology*
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Superoxide Dismutase / metabolism
Substances
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Alkaloids
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Amyloid beta-Peptides
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Cholinesterase Inhibitors
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Neuroprotective Agents
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Peptide Fragments
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Sesquiterpenes
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amyloid beta-protein (25-35)
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huperzine A
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Malondialdehyde
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Catalase
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Glutathione Peroxidase
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Superoxide Dismutase