Protective effects of huperzine A on beta-amyloid(25-35) induced oxidative injury in rat pheochromocytoma cells

Neurosci Lett. 2000 Jun 9;286(3):155-8. doi: 10.1016/s0304-3940(00)01088-0.

Abstract

The effects of huperzine A (HupA), a novel acetylcholinesterase inhibitor, on Abeta(25-35)-induced cell lesion, level of lipid peroxidation, antioxidant enzyme activities were investigated in the rat pheochromocytoma line PC12. Following a 48 h exposure of the cells to Abeta(25-35), a significant reduction in cell survival and activities of glutathione peroxidase (GSH-Px) and catalase (CAT), as well as increased production of malondialdehyde (MDA) and superoxide dismutase (SOD) were observed. Preincubation of the cells with HupA prior to Abeta(25-35) exposure elevated the cell survival and GSH-Px and CAT activities, and decreased the level of MDA and SOD activity. The results indicate that HupA has protective effects against Abeta-induced cell toxicity, which might be beneficial for the treatment of Alzheimer's disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaloids
  • Amyloid beta-Peptides / pharmacology*
  • Animals
  • Catalase / antagonists & inhibitors
  • Catalase / metabolism
  • Cell Survival / drug effects
  • Cholinesterase Inhibitors / pharmacology*
  • Glutathione Peroxidase / antagonists & inhibitors
  • Glutathione Peroxidase / metabolism
  • Malondialdehyde / metabolism
  • Neuroprotective Agents / pharmacology*
  • Oxidative Stress / physiology*
  • PC12 Cells / drug effects*
  • PC12 Cells / enzymology
  • PC12 Cells / pathology*
  • PC12 Cells / physiology
  • Peptide Fragments / pharmacology*
  • Rats
  • Sesquiterpenes / pharmacology*
  • Superoxide Dismutase / metabolism

Substances

  • Alkaloids
  • Amyloid beta-Peptides
  • Cholinesterase Inhibitors
  • Neuroprotective Agents
  • Peptide Fragments
  • Sesquiterpenes
  • amyloid beta-protein (25-35)
  • huperzine A
  • Malondialdehyde
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase