The exbD2 gene as well as the iron-uptake genes tonB, exbB and exbD1 of Xanthomonas campestris pv. campestris are essential for the induction of a hypersensitive response on pepper (Capsicum annuum)

Microbiology (Reading). 2000 May;146 ( Pt 5):1053-1060. doi: 10.1099/00221287-146-5-1053.

Abstract

The tonB, exbB and exbD1 genes of Xanthomonas campestris pv. campestris are essential for ferric iron uptake. In contrast, the exbD2 gene located in the same gene cluster is not essential. Mutational analysis revealed that the ferric-iron-uptake genes tonB, exbB and exbD1 are necessary for the induction of a hypersensitive response (HR) on the nonhost plant pepper (Capsicum annuum) and the induction of typical black rot symptoms on the host plant cauliflower (Brassica oleracea). Again, the exbD2 gene behaved differently. It was found to play a role only in the induction of the HR in pepper but not in the induction of black rot symptoms in cauliflower. Due to the low iron concentration in the plant tissue, the titre of viable bacteria of the ferric-iron-uptake mutants tonB, exbB and exbD1 decreased after leaf infiltration of pepper. The exbD2 mutant, however, which is not impaired in ferric iron uptake, multiplied in the pepper leaf tissue and grew even better than the wild-type strain, probably due to its failure to induce the HR. Nevertheless, the tonB, exbB and exbD1 mutant strains were able to spread systemically in cauliflower.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Brassica / microbiology
  • Capsicum / microbiology*
  • Escherichia coli Proteins*
  • Genes, Bacterial*
  • Membrane Proteins / genetics*
  • Mutation
  • Plant Diseases / microbiology*
  • Plants, Medicinal*
  • Xanthomonas campestris / chemistry
  • Xanthomonas campestris / genetics*
  • Xanthomonas campestris / pathogenicity

Substances

  • Bacterial Proteins
  • Escherichia coli Proteins
  • ExbB protein, E coli
  • Membrane Proteins
  • tonB protein, Bacteria
  • tonB protein, E coli
  • exbD protein, E coli