Dietary Bifidobacterium lactis (HN019) enhances resistance to oral Salmonella typhimurium infection in mice

Microbiol Immunol. 2000;44(4):213-22. doi: 10.1111/j.1348-0421.2000.tb02486.x.


The ability of a newly identified probiotic lactic acid bacterial strain, Bifidobacterium lactis (HN019), to confer protection against Salmonella typhimurium was investigated in BALB/c mice. Feeding mice with B. lactis conferred a significant degree of protection against single or multiple oral challenge with virulent S. typhimurium, in comparison to control mice that did not receive B. lactis. Protection included a ten-fold increase in survival rate, significantly higher post-challenge food intake and weight gain, and reduced pathogen translocation to visceral tissues (spleen and liver). Furthermore, the degree of pathogen translocation showed a significant inverse correlation with splenic lymphocyte proliferative responses to mitogens, blood and peritoneal cell phagocytic activity and intestinal mucosal anti-S. typhimurium antibody titers in infected mice; all of these immune parameters were enhanced in mice fed B. lactis. Together, these results suggest that dietary B. lactis can provide a significant degree of protection against Salmonella infection by enhancing various parameters of immune function that are relevant to the immunological control of salmonellosis. Thus dietary supplementation with B. lactis provides a unique opportunity for developing immune-enhancing probiotic dairy food products with proven health benefits.

MeSH terms

  • Animals
  • Antibodies, Bacterial / blood
  • Bacterial Translocation
  • Bifidobacterium*
  • Diet
  • Liver / microbiology
  • Lymphocyte Activation
  • Lymphocyte Subsets / immunology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Phagocytosis
  • Probiotics*
  • Salmonella Infections, Animal / immunology
  • Salmonella Infections, Animal / microbiology
  • Salmonella Infections, Animal / prevention & control*
  • Salmonella typhimurium / immunology
  • Salmonella typhimurium / pathogenicity
  • Salmonella typhimurium / physiology*
  • Spleen / microbiology
  • Virulence


  • Antibodies, Bacterial