Neurocan Is a Heparin Binding Proteoglycan

Biochem Biophys Res Commun. 2000 Jun 7;272(2):449-55. doi: 10.1006/bbrc.2000.2823.

Abstract

Neurocan and brevican are related chondroitin sulfate proteoglycans which are mainly expressed in the central nervous system. Neurocan and the secreted brevican variant are composed of globular N-terminal hyaluronan binding domains, central O-linked oligosaccharide attachment regions, and globular C-terminal domains. Interaction studies of mouse brain proteoglycans revealed that neurocan, but not brevican, was retained on a heparin affinity matrix. Also a recombinantly produced C-terminal fragment of neurocan, expressed by HEK 293 cells, was retained by the heparin affinity matrix. The substitution of this fragment with a chondroitin sulfate chain did not inhibit binding to the heparin affinity matrix at physiological NaCl concentrations, but decreased the NaCl concentration necessary for elution. Two potential consequences of the heparin binding ability of neurocan are an enforcement of the interaction with other heparin binding molecules and a directed secretion by polarized cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites
  • Blotting, Western
  • Brain
  • Brevican
  • Cell Line
  • Cell Polarity
  • Chondroitin Sulfate Proteoglycans / chemistry
  • Chondroitin Sulfate Proteoglycans / genetics
  • Chondroitin Sulfate Proteoglycans / isolation & purification
  • Chondroitin Sulfate Proteoglycans / metabolism*
  • Chromatography, Affinity
  • Dogs
  • Glycosylation
  • Heparin / metabolism*
  • Heparin / pharmacology
  • Humans
  • Hydrogen-Ion Concentration
  • Lectins, C-Type
  • Mice
  • Molecular Weight
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / isolation & purification
  • Nerve Tissue Proteins / metabolism*
  • Peptide Fragments / chemistry
  • Peptide Fragments / genetics
  • Peptide Fragments / isolation & purification
  • Peptide Fragments / metabolism
  • Protein Binding / drug effects
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Tenascin / chemistry
  • Tenascin / metabolism
  • Transfection

Substances

  • BCAN protein, human
  • Bcan protein, mouse
  • Brevican
  • Chondroitin Sulfate Proteoglycans
  • Lectins, C-Type
  • Nerve Tissue Proteins
  • Peptide Fragments
  • Recombinant Proteins
  • Tenascin
  • NCAN protein, human
  • Heparin