Full-length cDNA cloning and genomic organization of the mouse liver-specific organic anion transporter-1 (lst-1)

Biochem Biophys Res Commun. 2000 Jun 7;272(2):563-70. doi: 10.1006/bbrc.2000.2830.


We have cloned a cDNA that codes for mouse liver-specific transporter-1, mouse lst-1. The cDNA is comprised of 3296 base pairs and it contains a coding sequence for a protein of 689 amino acids with 12 putative transmembrane domains. The deduced amino acid sequence of the mouse lst-1 shares 64 and 77% identities with the reported human and rat lsts, respectively. Northern blot analysis demonstrates that mouse lst-1 mRNA is expressed exclusively in liver. We also report here the structural organization of the mouse lst-1 gene as the first evidence for the structure of a gene encoding an lst. The mouse lst-1 gene spans approximately 60 kbp in length and consists of 16 exons, including two noncoding exons. All the introns are flanked by GT-AG consensus splice sequences. 5'-Rapid Amplification of cDNA Ends (RACE) analyses demonstrate three splice variant mRNAs involving the noncoding exon 2 and exon 3. The 5'-flanking region of the gene contains consensus CAAT and TATA boxes and several potential binding sites for transcription factors for CAAT enhancer binding protein (C/EBP) and hepatocyte nuclear factors (HNF-3beta, HFH-1, and HFH-2), transcription factors important for liver-specific gene expression.

MeSH terms

  • 5' Untranslated Regions / genetics
  • Alternative Splicing / genetics
  • Amino Acid Sequence
  • Animals
  • Anion Transport Proteins
  • Base Sequence
  • CCAAT-Enhancer-Binding Proteins
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • DNA-Binding Proteins / metabolism
  • Exons / genetics*
  • Humans
  • Introns / genetics*
  • Liver / metabolism*
  • Mice
  • Molecular Sequence Data
  • Nuclear Proteins / metabolism
  • Organ Specificity
  • Phylogeny
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Response Elements / genetics
  • Sequence Alignment
  • TATA Box / genetics
  • Transcription Factors / metabolism


  • 5' Untranslated Regions
  • Anion Transport Proteins
  • CCAAT-Enhancer-Binding Proteins
  • Carrier Proteins
  • DNA, Complementary
  • DNA-Binding Proteins
  • Nuclear Proteins
  • RNA, Messenger
  • Transcription Factors

Associated data

  • GENBANK/AB031959