Unravelling genetic data by arrayed primer extension

Clin Chem Lab Med. 2000 Feb;38(2):165-70. doi: 10.1515/CCLM.2000.025.


We have developed a method for arrayed primer extension (APEX) on an oligonucleotide microchip together with the 4-color fluoresence imaging equipment and supporting software, that allows analysis of the DNA sequence and changes in it. Mutation analysis of BRCA1 gene and single nucleotide polymorphism (SNP) chip for genotyping were used as a model system. Chip surface chemistry, template preparation and APEX reaction conditions were optimised and the assay is ready to be implemented in variety of DNA analysis from SNP testing to DNA resequencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Base Sequence
  • DNA Mutational Analysis / methods
  • DNA Primers* / genetics
  • Female
  • Genes, BRCA1
  • Humans
  • Oligonucleotide Array Sequence Analysis* / instrumentation
  • Oligonucleotide Array Sequence Analysis* / methods
  • Polymorphism, Single Nucleotide


  • DNA Primers