Isolation of two novel genes, down-regulated in gastric cancer

Jpn J Cancer Res. 2000 May;91(5):459-63. doi: 10.1111/j.1349-7006.2000.tb00967.x.


Using a differential display technique, we identified two genes that are down-regulated in human gastric cancer tissue as compared to normal gastric mucosa. The down-regulated expression of these genes in gastric cancer tissue was confirmed by northern blotting analysis and RT-PCR. One, CA11, was a novel gene expressed predominantly in the stomach and was depleted in all of the gastric cancer cell lines examined. The other gene, GC36, was homologous to the digestive tract-specific calpain gene, nCL-4. The expression of both GC36 and nCL-4 was suppressed or depleted in gastric cancer cell lines of differentiated and poorly differentiated types. This is the first report of genes, the expression of which is down-regulated with considerable frequency in gastric cancer.

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Calpain / genetics*
  • Calpain / metabolism
  • Chromosomes, Human, Pair 1 / genetics
  • Chromosomes, Human, Pair 2 / genetics
  • DNA, Complementary / isolation & purification
  • DNA, Complementary / metabolism
  • DNA, Neoplasm / isolation & purification
  • DNA, Neoplasm / metabolism
  • Down-Regulation
  • Gastric Mucosa / metabolism
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Isoenzymes / genetics
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics*
  • Neoplasm Proteins / metabolism
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / metabolism
  • Tumor Cells, Cultured


  • DNA, Complementary
  • DNA, Neoplasm
  • Isoenzymes
  • Neoplasm Proteins
  • RNA, Messenger
  • Calpain

Associated data

  • GENBANK/AB038463
  • GENBANK/AB039886