Immunoradiometric assay of factor VIII related antigen, with observations in 32 patients with von Willebrand's disease

Br J Haematol. 1976 Jun;33(2):221-32. doi: 10.1111/j.1365-2141.1976.tb03533.x.

Abstract

A solid phase non-competitive immunoradiometric assay (IRMA) has been developed which allows measurement of factor VIII related antigen (VIIIR:AG) levels in normal plasma as low as 2.5 x 10(-4) U/ml. The assay is based on the extraction of VIIIR:AG from test plasma by means of polystrene tubes coated with a specific unlabelled anti-VIIIR:AG rabbit antiserum and subsequent labelling of the extracted antigen with 125I-labelled anti-VIIIR:AG rabbit IgG.

MeSH terms

  • Antibody Specificity
  • Antigens / analysis*
  • Dose-Response Relationship, Drug
  • Factor VIII / immunology*
  • Female
  • Humans
  • Male
  • Radioimmunoassay / methods
  • Time Factors
  • von Willebrand Diseases / genetics
  • von Willebrand Diseases / immunology*

Substances

  • Antigens
  • Factor VIII