Detection of extended-spectrum beta-lactamases in members of the family enterobacteriaceae: comparison of the MAST DD test, the double disc and the Etest ESBL

J Antimicrob Chemother. 2000 Jun;45(6):881-5. doi: 10.1093/jac/45.6.881.

Abstract

A technically simple method-the MAST double disc (MDD) test-for the detection of extended-spectrum beta-lactamase (ESBL) production by bacteria is described. A wide range of ESBL, non-ESBL and Class 1 beta-lactamase-producing isolates was examined. The MDD test, which uses discs containing ceftazidime and a complementary disc containing ceftazidime and clavulanate and a second pair containing cefotaxime and cefotaxime and clavulanate was compared with the standard double disc diffusion test and an Etest method. Both the Etest and the MDD correctly identified 93% of ESBL producers. The MDD is an inexpensive alternative to current methods for the detection of ESBL production.

Publication types

  • Comparative Study

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Ceftazidime / pharmacology
  • Cephalosporins / pharmacology
  • Clavulanic Acid / pharmacology
  • Enterobacteriaceae / drug effects
  • Enterobacteriaceae / enzymology*
  • Genes, Bacterial / genetics
  • Microbial Sensitivity Tests
  • Reverse Transcriptase Polymerase Chain Reaction
  • beta-Lactamases / metabolism*

Substances

  • Anti-Bacterial Agents
  • Cephalosporins
  • Clavulanic Acid
  • Ceftazidime
  • beta-Lactamases