Full length alpha-synuclein is present in cerebrospinal fluid from Parkinson's disease and normal subjects

Neurosci Lett. 2000 Jun 16;287(1):65-7. doi: 10.1016/s0304-3940(00)01153-8.


Several clues suggest that alpha-synuclein, a presynaptic protein, plays a central role in the pathogenesis of idiopathic Parkinson's disease (PD). To search a peripheral marker of PD, we analyzed presence and amount of alpha-synuclein in CSF from 12 PD patients and 10 neurologically normal subjects. The protein was extracted from CSF samples through immunoprecipitation and immunoblotting with different specific anti-alpha-synuclein antibodies. We identified a 19 kDa band that corresponds to monomeric alpha-synuclein, given its comigration with homologue human recombinant peptide as well as with the protein extracted from cerebral cortex of normal subjects. The amount of CSF 19 kDa alpha-synuclein did not significantly vary in PD and normal cases. These findings have two implications: (a) full length alpha-synuclein is released by neurons in the extracellular space; (b) alpha-synuclein does not appear a peripheral marker of PD pathology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers / cerebrospinal fluid
  • Humans
  • Immunoblotting
  • Nerve Tissue Proteins / cerebrospinal fluid*
  • Parkinson Disease / cerebrospinal fluid*
  • Synucleins
  • alpha-Synuclein


  • Biomarkers
  • Nerve Tissue Proteins
  • SNCA protein, human
  • Synucleins
  • alpha-Synuclein