A role for potassium in TNF-induced apoptosis and gene-induction in human and rodent tumour cell lines

Cytokine. 2000 Jun;12(6):747-50. doi: 10.1006/cyto.1999.0626.

Abstract

Rat/mouse T cell hybridoma-derived PC60 R55/R75 cells were used as a model to study the role of intracellular potassium in TNF-induced apoptosis and gene induction. A reduction of intracellular potassium with nigericin or valinomycin (ionophores), or ouabain (Na(+)/K(+)-ATPase inhibitor) sensitized PC60 R55/R75 cells to TNF-induced apoptosis. TNF-induced GM-CSF release in PC60 R55/R75 cells was enhanced by nigericin or ouabain. Similar results were obtained with human cervix carcinoma cells HeLaH21 exposed to TNF. These results suggest a role for intracellular potassium in TNF-induced apoptosis and gene induction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Apoptosis / physiology*
  • Cell Survival / drug effects
  • Gene Expression Regulation, Neoplastic / drug effects
  • Gene Expression Regulation, Neoplastic / physiology*
  • Granulocyte-Macrophage Colony-Stimulating Factor / genetics*
  • HeLa Cells
  • Humans
  • Hybridomas
  • Mice
  • Nigericin / pharmacology
  • Ouabain / pharmacology
  • Potassium / physiology*
  • Recombinant Proteins / pharmacology
  • T-Lymphocytes
  • Transcriptional Activation
  • Tumor Necrosis Factor-alpha / pharmacology*
  • Valinomycin / pharmacology

Substances

  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • Valinomycin
  • Ouabain
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Nigericin
  • Potassium