Comedogenicity of squalene monohydroperoxide in the skin after topical application

J Toxicol Sci. 2000 May;25(2):77-83. doi: 10.2131/jts.25.77.


The comedogenicity of squalene peroxides was examined on the rabbit ear skin after topical application of squalene-monohydroperoxide (Sq-OOH), the initial product when squalene was irradiated with UV-A. Since comedogenic products from UV-irradiated squalene were extracted with methanol solution, we isolated Sq-OOH by reverse-phased HPLC with a methanol mobile phase solvent. The degree of comedogenic reaction induced by Sq-OOH was higher than that of well-known comedogenic cosmetic ingredients. Unlike two other mono-peroxides, tert-butyl hydroperoxide and cumene-mono-hydroperoxide, Sq-OOH induced comedo-formation in the rabbit ear skin. However, the comedogenicity of reduced Sq-OOH, squalene-hydroxide (Sq-OH) and squalene itself was lower than that of Sq-OOH. These results indicate that Sq-OOH is a potent comedogenic mono-hydroperoxide chemical to rabbit skin.

Publication types

  • Comparative Study

MeSH terms

  • Acne Vulgaris / chemically induced*
  • Acne Vulgaris / pathology
  • Administration, Topical
  • Animals
  • Chromatography, High Pressure Liquid
  • Dose-Response Relationship, Drug
  • Ear, External / drug effects
  • Ear, External / pathology
  • Hydrogen Peroxide / administration & dosage
  • Hydrogen Peroxide / chemical synthesis
  • Hydrogen Peroxide / toxicity*
  • Lipid Peroxides / toxicity
  • Male
  • Photolysis
  • Rabbits
  • Skin / drug effects*
  • Skin / pathology
  • Squalene / administration & dosage
  • Squalene / analogs & derivatives
  • Squalene / chemical synthesis
  • Squalene / radiation effects
  • Squalene / toxicity*
  • Toxicity Tests
  • Ultraviolet Rays


  • Lipid Peroxides
  • Squalene
  • Hydrogen Peroxide