Functional analysis of tobacco LIM protein Ntlim1 involved in lignin biosynthesis

Plant J. 2000 May;22(4):289-301. doi: 10.1046/j.1365-313x.2000.00737.x.

Abstract

The AC-rich motif, Pal-box, is an important cis-acting element for gene expression involved in phenylpropanoid biosynthesis. A cDNA clone (Ntlim1) encoding a Pal-box binding protein was isolated by Southwestern screening. The deduced amino acid sequence is highly similar to the members of the LIM protein family that contain a zinc finger motif. Moreover, Ntlim1 had a specific DNA binding ability and transiently activated the transcription of a beta-glucuronidase reporter gene driven by the Pal-box sequence in tobacco protoplasts. The transgenic tobacco plants with antisense Ntlim1 showed low levels of transcripts from some key phenylpropanoid pathway genes such as phenylalanine ammonia-lyase, hydroxycinnamate CoA ligase and cinnamyl alcohol dehydrogenase. Furthermore, a 27% reduction of lignin content was observed in the transgenic tobacco with antisense Ntlim1.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA, Complementary
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism*
  • Lignin / biosynthesis*
  • Molecular Sequence Data
  • Phenylpropionates / metabolism
  • Plants, Genetically Modified
  • Plants, Toxic*
  • RNA, Messenger / genetics
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Tobacco / metabolism*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • DNA, Complementary
  • Homeodomain Proteins
  • Phenylpropionates
  • RNA, Messenger
  • Transcription Factors
  • Lignin

Associated data

  • GENBANK/AB023479