Ozone treatment rapidly activates MAP kinase signalling in plants

Plant J. 2000 May;22(4):367-76. doi: 10.1046/j.1365-313x.2000.00741.x.

Abstract

Brief exposure to ozone, a potent cross-inducer of plant stress responses, leads within minutes to activation of an ERK-type MAP kinase (approximately 46 kDa) in tobacco. This activation process is calcium-dependent and can be blocked both by free radical quenchers and by a specific inhibitor of MEK-1 (MAPKK). Hydrogen peroxide and superoxide anion radicals can substitute for ozone as the activation stimulus, which does not appear to require salicylate as an intermediary. The properties of the ozone-induced MAPK suggest that it may be SIPK (salicylate-induced protein kinase), a tobacco MAPK that is activated by a variety of stress treatments. The ability of ozone to activate SIPK indicates that this protein kinase acts as a very early transducer of redox stress signals in plant cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium / metabolism
  • Cells, Cultured
  • Enzyme Activation
  • MAP Kinase Signaling System / drug effects*
  • Mitogen-Activated Protein Kinases / metabolism
  • Ozone / pharmacology*
  • Plants / drug effects*
  • Plants / enzymology
  • Plants / metabolism
  • Reactive Oxygen Species
  • Salicylic Acid / metabolism

Substances

  • Reactive Oxygen Species
  • Ozone
  • Mitogen-Activated Protein Kinases
  • Salicylic Acid
  • Calcium