Review of recent developments in the molecular characterization of recombinant alfa interferons on the 40th anniversary of the discovery of interferon

Cancer Biother Radiopharm. 1998 Jun;13(3):143-54. doi: 10.1089/cbr.1998.13.143.


Recombinant alfa interferons (IFN-alpha s) are approved worldwide for the treatment of a variety of cancers and diseases of virologic origin. A series of recent advances in the molecular characterization of recombinant IFN-alpha s have allowed the determination of the three-dimensional IFN-alpha 2b structure by high-resolution x-ray crystallography. We review here recent developments in our understanding of the molecular and physicochemical properties of recombinant IFN-alpha, including our current state of knowledge of the IFN-alpha gene family and the multiple species of human leukocyte IFN. Based on the reported three-dimensional structure of IFN-alpha 2b, we propose a molecular model for the IFN-alpha 2b receptor complex and predict models for the naturally occurring subtypes IFN-alpha 1 and IFN-alpha 8, as well as the synthetic, non-naturally occurring consensus IFN. Such models provide molecular insights into the mechanism of action of IFN-alpha.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Humans
  • Interferon Type I / chemistry*
  • Interferon Type I / therapeutic use*
  • Interferon alpha-2
  • Interferon-alpha / chemistry
  • Interferon-alpha / genetics
  • Models, Molecular
  • Neoplasms / drug therapy*
  • Protein Conformation
  • Receptors, Interferon / chemistry
  • Recombinant Proteins
  • Virus Diseases / drug therapy*


  • Interferon Type I
  • Interferon alpha-2
  • Interferon-alpha
  • Receptors, Interferon
  • Recombinant Proteins