Phytohaemagglutinin stimulation of rat thymus lymphocytes glycolysis

Biochim Biophys Acta. 1976 Jul 21;437(2):354-63. doi: 10.1016/0304-4165(76)90005-2.

Abstract

Glucose disappearance and lactate production by the rat thymocytes are stimulated significantly 45 min after addition of phytohaemagglutinin or concanavalin A and the stimulated rate is sustained for at least 8 h. Changes in the steady-state concentration of glycolytic intermediates that occur at non-equilibrium steps during the increased rate of glycolytic flux indicate that the glucose carrier, hexokinase and phosphofructokinase are potentially regulatory steps that undergo nearly simultaneous or tightly sequential activation following interaction of the cells with the mitogen.

MeSH terms

  • Adenine Nucleotides / metabolism
  • Animals
  • Concanavalin A / pharmacology*
  • Dose-Response Relationship, Drug
  • Glucose / metabolism
  • Glycolysis / drug effects*
  • Kinetics
  • Lactates / metabolism
  • Lectins / pharmacology*
  • Oxygen Consumption / drug effects
  • Rats
  • Species Specificity
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / metabolism*
  • Thymidine / metabolism

Substances

  • Adenine Nucleotides
  • Lactates
  • Lectins
  • Concanavalin A
  • Glucose
  • Thymidine