Establishment of a human colon cancer cell line (PMF-ko14) displaying highly metastatic activity

Int J Oncol. 2000 Jul;17(1):39-45. doi: 10.3892/ijo.17.1.39.


A new human colon cancer cell line (PMF-ko14) derived from a peritoneal disseminated tumor has been established and maintained for over 25 months. In tissue culture, the cells grew in a mainly monolayered sheet with a population doubling time of about 27 h. Chromosome counts at the 60th passage ranged from 79 to 84 with a modal number of 83. Flow cytometry of the cell surface antigen expression indicated that CD49b, CD29, carcinoembryonic antigen (CEA), sialyl Lewis a (sLea), and CD49c were positive in more than 70% of the cells. The nude mouse xenograft models indicated are: subcutaneous or intraperitoneal injection model, spleen injection-liver metastasis model, and orthotopic implantation-spontaneous metastasis model. As PMF-ko14 has highly metastatic activity it should prove to be a useful tool for research in biological behavior of metastatic colon cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD / analysis
  • Cell Culture Techniques / methods
  • Cell Division
  • Chromosome Banding
  • Colonic Neoplasms / genetics
  • Colonic Neoplasms / pathology*
  • Flow Cytometry
  • Humans
  • Karyotyping
  • Kinetics
  • Liver Neoplasms / pathology
  • Liver Neoplasms / secondary*
  • Mice
  • Mice, Nude
  • Neoplasm Metastasis*
  • Peritoneal Neoplasms / pathology
  • Peritoneal Neoplasms / secondary
  • Transplantation, Heterologous
  • Tumor Cells, Cultured


  • Antigens, CD