Regeneration of halved embryonic lower first mouse molars: correlation with the distribution pattern of non dividing IDE cells, the putative organizers of morphogenetic units, the cusps

Int J Dev Biol. 2000 Apr;44(3):289-95.


Recently we demonstrated that non-cycling, cap-stage, mouse molar inner dental epithelial (IDE) cells corresponding to the primary enamel knot (EK) area underwent a coordinated temporo-spatial patterning leading to their patchy irregular segregation at the tips of the forming cusps. These non-cycling cells were suggested to perhaps represent the organizers of the morphogenetic units (OMU), the cusps. The present study has analyzed the regenerative capacity of halved cap-stage first lower mouse molars through three dimensional (3D) reconstructions. Partial regeneration of the anterior half and possible complete regeneration of the posterior half were documented. Using BrdU (5-bromo-2'-deoxyuridine) labeling and 3D reconstructions of the IDE, we have correlated the patterns of cusp regeneration with the distribution of BrdU negative IDE cells. These data support a morphogenetic role for the non-cycling IDE cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bromodeoxyuridine / metabolism
  • Cuspid / embryology*
  • Image Processing, Computer-Assisted
  • Immunohistochemistry
  • Mice
  • Mice, Inbred ICR
  • Microscopy, Electron, Scanning
  • Molar / embryology*
  • Organ Culture Techniques
  • Regeneration
  • Time Factors


  • Bromodeoxyuridine