Monitoring antigen-specific T cells using MHC-Ig dimers

Immunol Invest. 2000 May;29(2):163-9. doi: 10.3109/08820130009062300.

Abstract

To summarize, two novel approaches are currently being examined that allow for identification of antigen-specific T cells. A biochemical approach to generating soluble multivalent MHC complexes has been to generate tetrameric MHC complexes linked to avidin. We have also generated a general approach for producing soluble divalent versions of class I and class II MHC molecules, using Ig as a molecular scaffold. The experimental system described here outlines a general approach of using multivalent high affinity ligands to study cell-cell interactions, driven by multivalent ligand-receptor interactions. Our work indicates that divalent chimeric molecules are high-avidity analogs of proteins useful in probing and selectively regulating cellular responses.

MeSH terms

  • Antigens / immunology*
  • Antigens, Surface / immunology
  • Dimerization
  • Histocompatibility Antigens Class I / chemistry
  • Histocompatibility Antigens Class I / immunology*
  • Histocompatibility Antigens Class II / chemistry
  • Histocompatibility Antigens Class II / immunology*
  • Humans
  • Immunoglobulins / chemistry
  • Immunoglobulins / immunology*
  • In Vitro Techniques
  • Protein Structure, Quaternary
  • T-Lymphocytes, Cytotoxic / immunology*

Substances

  • Antigens
  • Antigens, Surface
  • Histocompatibility Antigens Class I
  • Histocompatibility Antigens Class II
  • Immunoglobulins