A2-Pancortins (Pancortin-3 and -4) are the dominant pancortins during neocortical development

J Neurochem. 2000 Jul;75(1):1-8. doi: 10.1046/j.1471-4159.2000.0750001.x.


We have identified a novel mouse gene named pancortin that is expressed dominantly in the mature cerebral cortex. This gene produces four different species of proteins, Pancortin-1-4, sharing a common region in the middle of their structure with two variations at the N-terminal (A1 or A2 part) and C-terminal (C1 or C2 part) sides, respectively. In the present study, we showed that expression of mRNAs for A2-Pancortins (Pancortin species that contain the A2 part, i.e., Pancortin-3 and -4) is more dominant than that of mRNAs for A1-Pancortins (Pancortin species that contain the A1 part, i.e., Pancortin-1 and -2) in the prenatal mouse cerebral neocortex. Using western blot analysis, we found that substantial amounts of both A2-Pancortins were present in the prenatal cerebral neocortex and P19 cells after inducing neuronal differentiation. A2-Pancortins were still present in the cerebral neocortex of the adult, although their mRNAs were hardly detected. In contrast, the amount of A1-Pancortins did not increase after the third postnatal week in spite of their intense gene expression. Furthermore, we showed that recombinant Pancortin-3, one of the A2-Pancortins, was a secreted protein, in contrast to Pancortin-1 (one of the A1-Pancortins). These results suggest that A2-Pancortins are extracellular proteins essential for neuronal differentiation and that their molecular behavior is distinct from that of A1-Pancortins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / analysis
  • Amino Acid Sequence
  • Animals
  • Blotting, Western
  • COS Cells
  • Cell Differentiation
  • Cell Line
  • Extracellular Matrix Proteins
  • Female
  • Gene Expression*
  • Glycoproteins / analysis*
  • Glycoproteins / chemistry
  • Glycoproteins / genetics*
  • In Situ Hybridization
  • Mice
  • Mice, Inbred ICR
  • Molecular Sequence Data
  • Neocortex / chemistry
  • Neocortex / embryology
  • Neocortex / growth & development*
  • Nerve Tissue Proteins / analysis*
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / genetics*
  • Neurons / chemistry
  • Neurons / physiology
  • Peptide Fragments / chemistry
  • Peptide Fragments / immunology
  • RNA, Messenger / analysis
  • Rabbits
  • Transfection


  • Extracellular Matrix Proteins
  • Glycoproteins
  • Nerve Tissue Proteins
  • Peptide Fragments
  • RNA, Messenger
  • olfactomedin
  • Alkaline Phosphatase