Abstract
Anti-Müllerian hormone induces the regression of fetal Müllerian ducts and inhibits the transcription of gonadal steroidogenic enzymes. It belongs to the transforming growth factor-beta family whose members signal through a pair of serine/threonine kinase receptors and Smad effectors. Only the anti-Müllerian hormone type II receptor has been identified. Our goal was to determine whether anti-Müllerian hormone could share a type I receptor with another family member. Co-immunoprecipitation of known type I receptors with anti-Müllerian hormone type II receptor clearly showed that the bone morphogenetic protein type IB receptor was the only cloned type I receptor interacting in a ligand-dependent manner with this type II receptor. Anti-Müllerian hormone also activates the bone morphogenetic protein-specific Smad1 pathway and the XVent2 reporter gene, an anti-Müllerian hormone type II receptor-dependent effect abrogated by a dominant negative version of bone morphogenetic protein type IB receptor. Reverse amplification experiments showed that bone morphogenetic protein type IB receptor is co-expressed with anti-Müllerian hormone type II receptor in most anti-Müllerian hormone target tissues. Our data support a model in which a ligand, anti-Müllerian hormone, gains access to a shared type I receptor and Smad1 system through a highly restricted type II receptor.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Anti-Mullerian Hormone
-
Bone Morphogenetic Protein Receptors, Type I
-
Bone Morphogenetic Protein Receptors, Type II
-
CHO Cells
-
Cell Line
-
Cricetinae
-
DNA-Binding Proteins*
-
Genes, Reporter
-
Glycoproteins*
-
Growth Inhibitors / pharmacology*
-
Humans
-
Mice
-
Protein Serine-Threonine Kinases / drug effects
-
Protein Serine-Threonine Kinases / genetics
-
Protein Serine-Threonine Kinases / physiology*
-
Proteins / metabolism*
-
Receptors, Cell Surface / physiology
-
Receptors, Growth Factor / drug effects
-
Receptors, Growth Factor / genetics
-
Receptors, Growth Factor / physiology*
-
Receptors, Peptide / drug effects
-
Receptors, Peptide / genetics
-
Receptors, Peptide / physiology*
-
Receptors, Transforming Growth Factor beta
-
Recombinant Proteins / metabolism
-
Recombinant Proteins / pharmacology
-
Reverse Transcriptase Polymerase Chain Reaction
-
Signal Transduction / drug effects
-
Smad Proteins
-
Smad1 Protein
-
Testicular Hormones / pharmacology*
-
Trans-Activators*
-
Transfection
-
Tumor Cells, Cultured
Substances
-
DNA-Binding Proteins
-
Glycoproteins
-
Growth Inhibitors
-
Proteins
-
Receptors, Cell Surface
-
Receptors, Growth Factor
-
Receptors, Peptide
-
Receptors, Transforming Growth Factor beta
-
Recombinant Proteins
-
SMAD1 protein, human
-
Smad Proteins
-
Smad1 Protein
-
Smad1 protein, mouse
-
Testicular Hormones
-
Trans-Activators
-
anti-Mullerian hormone receptor
-
Anti-Mullerian Hormone
-
Protein Serine-Threonine Kinases
-
BMPR1B protein, human
-
BMPR2 protein, human
-
Bmpr1b protein, mouse
-
Bmpr2 protein, mouse
-
Bone Morphogenetic Protein Receptors, Type I
-
Bone Morphogenetic Protein Receptors, Type II