Immunodetection of adenylyl cyclase protein in tissues

Mol Cell Endocrinol. 2000 Apr 25;162(1-2):107-12. doi: 10.1016/s0303-7207(00)00210-0.

Abstract

Immunodetection of the adenylyl cyclase isoforms has been difficult in tissues because of its low quantity of protein expression. We have developed a one-step cellular protein purification method that enables a simple immunodetection of the adenylyl cyclase isoforms. The type I isoform was detected exclusively in the brain. The type III isoform was detected in the brain and lungs. Further, the protein expression of type III adenylyl cyclase in lungs changed ontogenically and was the lowest in neonates. Thus, the comparison of the amount of certain adenylyl cyclase isoforms protein in each tissue is now feasible using our method.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenylyl Cyclases / genetics
  • Adenylyl Cyclases / isolation & purification
  • Adenylyl Cyclases / metabolism*
  • Animals
  • Animals, Newborn
  • Brain / enzymology
  • Immunohistochemistry / methods*
  • Isoenzymes / genetics
  • Isoenzymes / isolation & purification
  • Isoenzymes / metabolism
  • Lung / enzymology
  • Lung / growth & development
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Swine
  • Tissue Distribution

Substances

  • Isoenzymes
  • RNA, Messenger
  • Adenylyl Cyclases