A cross-sectional study of paratuberculosis in 1155 Danish dairy cows

Prev Vet Med. 2000 Jul 3;46(1):15-27. doi: 10.1016/s0167-5877(00)00138-0.


In a cross-sectional study on milk samples from 1155 cows from 22 Danish dairy herds, selected risk factors for paratuberculosis were identified. The diagnostic procedure used was an indirect enzyme-linked immuno-sorbent assay (ELISA) to detect antibodies against Mycobacterium avium subsp. paratuberculosis. A sample was considered test-positive if it had a corrected optical density >/=0. 025 (test sensitivity 71.4% and test specificity 89.7%). Of the 1155 samples, 8.8% (102/1155) were test-positive, and 19 out of the 22 dairy herds had >/=1 test-positive cows. The significant risk factors in a multiple logistic regression analysis were: Jersey versus large breeds, high parity versus low parity, the first month after calving versus other months of lactation, and a large herd size compared to a small herd size. The highest probability (37-38%) of a positive test was observed among older cows (parity >4) and tested within the first month after calving (irrespective of breed). The lowest probability (2%) of a positive test-result was observed among first parity, large-breed cows tested before calving or later than one month after.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cross-Sectional Studies
  • Denmark / epidemiology
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Lactation
  • Milk / microbiology*
  • Mycobacterium avium subsp. paratuberculosis / isolation & purification*
  • Paratuberculosis / diagnosis
  • Paratuberculosis / epidemiology*
  • Paratuberculosis / etiology
  • Parity
  • Probability
  • Regression Analysis
  • Risk Factors
  • Seroepidemiologic Studies
  • Species Specificity